Recent Publication Highlights

Structure-based stabilization of SOSIP Env enhances recombinant ectodomain durability and yield

D. Wrapp, Z. Mu, B. Thakur, K. Janowska, O. Ajayi, M. Barr, R. Parks, K. Mansouri, R.J. Edwards, B.H. Hahn, P. Acharya, K.O. Saunders, B.F. Haynes

The Env glycoprotein is the main focus of HIV vaccine development but due to its inherent metastability, many Env variants are difficult to recombinantly express in the relatively large quantities that are required for biochemical studies and animal trials. Here, we describe a novel structure-based stabilization strategy that works synergistically with previously described SOSIP mutations to dramatically increase the yield of prefusion HIV-1 Env.

Selected Publications

Nanoparticle display of prefusion coronavirus spike elicits S1-focused cross-reactive antibody response against diverse coronavirus subgenera (PMID: 37794071)

Engineered immunogens to elicit antibodies against conserved coronavirus epitopes (PMID: 38036525)

A single, improbable B cell receptor mutation confers potent neutralization against cytomegalovirus (PMID: 36662906)

Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies (PMID: 35275718)

Vaccination with prefusion-stabilized respiratory syncytial virus fusion protein induces genetically and antigenically diverse antibody responses (PMID: 33823129)

Structure-based design of prefusion-stabilized SARS-CoV-2 spikes (PMID: 32703906)

Recognition of a highly conserved glycoprotein B epitope by a bivalent antibody neutralizing HCMV at a post-attachment step (PMID: 32745149)

Structural basis for potent neutralization of betacoronavirus by single-domain camelid antibodies (PMID: 32531248)

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation (PMID: 32075877)